Respiratory Enzymes

Respiratory Enzymes in Oxidative Phosphorylation

In a complex enzymatic sequence the site of action of substrates and inhibitors is clearly marked by the way they affect the steady state concentrations of the components of the system (1). Antimycin A, for example, inhibits respiration in the succinic oxidase system (2) and at the same time increases the steady state reduction of cytochrome b and decreases that of cytochromes c, a, and aa (3)....

The Respiratory and Glycolytic Enzymes

A number of workers have studied the carbohydrate metabolism of sea-urchin eggs and have expressed different opinions about the pathway of carbohydrate breakdown in these eggs. The choice has rested between the breakdown of hexose via the 'hexose monophosphate shunt' and a glycolytic mechanism like that in yeast and muscle, followed by oxidation via the tricarboxylic acid cycle. Rothschild (195...

The Effect of Sulfonamides on Respiratory Enzymes

In recent years it has been established that sulfonamides inhibit the respiration of certain bacteria, yeasts, and tissues. This inhibition was first observed on intact bacteria by Barron and Jacobs (l), and it seems to occur simultaneously with the cessation of growth in the presence of the sulfonamide (2). On the basis of numerous investigations, it can be assumed that certain relationships e...

Mimicking respiratory phosphorylation using purified enzymes.

The enzymes of oxidative phosphorylation is a striking example of the functional association of multiple enzyme complexes, working together to form ATP from cellular reducing equivalents. These complexes, such as cytochrome c oxidase or the ATP synthase, are typically investigated individually and therefore, their functional interplay is not well understood. Here, we present methodology that al...

The Terminal Respiratory Enzymes in Neurospora Tetrasperma.